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(A) Schematic diagram of the forward genetic screening to search for factors required for NoSB formation in C. elegans nuclei. (B) DIC and fluorescence microscopy images of nuclei in the indicated animals. (C) Schematic of the nosr-1 exon structure. The deletion alleles ust307 , ust308 and ust309 were constructed by <t>CRISPR/Cas9</t> technology. (D) Quantification of NoSB in hypodermic cells in the indicated animals. Every dot indicates the percentage of hypodermis cells containing NoSB in each worm. n>19 animals. (E) Images of mCherry::DIS-3 transgene in nosr-1 ( - ) ;rpl-14(RNAi) animals. (F) Quantification of nucleolar rings and NoSB in hypodermic cells in the indicated animals. Every dot indicates the percentage of hypodermis cells containing NoSB in each worm.
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(A) Schematic diagram of the forward genetic screening to search for factors required for NoSB formation in C. elegans nuclei. (B) DIC and fluorescence microscopy images of nuclei in the indicated animals. (C) Schematic of the nosr-1 exon structure. The deletion alleles ust307 , ust308 and ust309 were constructed by <t>CRISPR/Cas9</t> technology. (D) Quantification of NoSB in hypodermic cells in the indicated animals. Every dot indicates the percentage of hypodermis cells containing NoSB in each worm. n>19 animals. (E) Images of mCherry::DIS-3 transgene in nosr-1 ( - ) ;rpl-14(RNAi) animals. (F) Quantification of nucleolar rings and NoSB in hypodermic cells in the indicated animals. Every dot indicates the percentage of hypodermis cells containing NoSB in each worm.
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(A) Schematic diagram of the forward genetic screening to search for factors required for NoSB formation in C. elegans nuclei. (B) DIC and fluorescence microscopy images of nuclei in the indicated animals. (C) Schematic of the nosr-1 exon structure. The deletion alleles ust307 , ust308 and ust309 were constructed by <t>CRISPR/Cas9</t> technology. (D) Quantification of NoSB in hypodermic cells in the indicated animals. Every dot indicates the percentage of hypodermis cells containing NoSB in each worm. n>19 animals. (E) Images of mCherry::DIS-3 transgene in nosr-1 ( - ) ;rpl-14(RNAi) animals. (F) Quantification of nucleolar rings and NoSB in hypodermic cells in the indicated animals. Every dot indicates the percentage of hypodermis cells containing NoSB in each worm.
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(A) Schematic diagram of the forward genetic screening to search for factors required for NoSB formation in C. elegans nuclei. (B) DIC and fluorescence microscopy images of nuclei in the indicated animals. (C) Schematic of the nosr-1 exon structure. The deletion alleles ust307 , ust308 and ust309 were constructed by <t>CRISPR/Cas9</t> technology. (D) Quantification of NoSB in hypodermic cells in the indicated animals. Every dot indicates the percentage of hypodermis cells containing NoSB in each worm. n>19 animals. (E) Images of mCherry::DIS-3 transgene in nosr-1 ( - ) ;rpl-14(RNAi) animals. (F) Quantification of nucleolar rings and NoSB in hypodermic cells in the indicated animals. Every dot indicates the percentage of hypodermis cells containing NoSB in each worm.
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(A) Schematic diagram of the forward genetic screening to search for factors required for NoSB formation in C. elegans nuclei. (B) DIC and fluorescence microscopy images of nuclei in the indicated animals. (C) Schematic of the nosr-1 exon structure. The deletion alleles ust307 , ust308 and ust309 were constructed by <t>CRISPR/Cas9</t> technology. (D) Quantification of NoSB in hypodermic cells in the indicated animals. Every dot indicates the percentage of hypodermis cells containing NoSB in each worm. n>19 animals. (E) Images of mCherry::DIS-3 transgene in nosr-1 ( - ) ;rpl-14(RNAi) animals. (F) Quantification of nucleolar rings and NoSB in hypodermic cells in the indicated animals. Every dot indicates the percentage of hypodermis cells containing NoSB in each worm.
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(A) Schematic diagram of the forward genetic screening to search for factors required for NoSB formation in C. elegans nuclei. (B) DIC and fluorescence microscopy images of nuclei in the indicated animals. (C) Schematic of the nosr-1 exon structure. The deletion alleles ust307 , ust308 and ust309 were constructed by <t>CRISPR/Cas9</t> technology. (D) Quantification of NoSB in hypodermic cells in the indicated animals. Every dot indicates the percentage of hypodermis cells containing NoSB in each worm. n>19 animals. (E) Images of mCherry::DIS-3 transgene in nosr-1 ( - ) ;rpl-14(RNAi) animals. (F) Quantification of nucleolar rings and NoSB in hypodermic cells in the indicated animals. Every dot indicates the percentage of hypodermis cells containing NoSB in each worm.
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Image Search Results


(A) Schematic diagram of the forward genetic screening to search for factors required for NoSB formation in C. elegans nuclei. (B) DIC and fluorescence microscopy images of nuclei in the indicated animals. (C) Schematic of the nosr-1 exon structure. The deletion alleles ust307 , ust308 and ust309 were constructed by CRISPR/Cas9 technology. (D) Quantification of NoSB in hypodermic cells in the indicated animals. Every dot indicates the percentage of hypodermis cells containing NoSB in each worm. n>19 animals. (E) Images of mCherry::DIS-3 transgene in nosr-1 ( - ) ;rpl-14(RNAi) animals. (F) Quantification of nucleolar rings and NoSB in hypodermic cells in the indicated animals. Every dot indicates the percentage of hypodermis cells containing NoSB in each worm.

Journal: bioRxiv

Article Title: Nucleolar stress induced the formation of a nucleolar stress body via the NOSR-1/NUMR-1 axis in Caenorhabditis elegans

doi: 10.1101/2024.03.05.583293

Figure Lengend Snippet: (A) Schematic diagram of the forward genetic screening to search for factors required for NoSB formation in C. elegans nuclei. (B) DIC and fluorescence microscopy images of nuclei in the indicated animals. (C) Schematic of the nosr-1 exon structure. The deletion alleles ust307 , ust308 and ust309 were constructed by CRISPR/Cas9 technology. (D) Quantification of NoSB in hypodermic cells in the indicated animals. Every dot indicates the percentage of hypodermis cells containing NoSB in each worm. n>19 animals. (E) Images of mCherry::DIS-3 transgene in nosr-1 ( - ) ;rpl-14(RNAi) animals. (F) Quantification of nucleolar rings and NoSB in hypodermic cells in the indicated animals. Every dot indicates the percentage of hypodermis cells containing NoSB in each worm.

Article Snippet: Addgene plasmid #47549 was used to express Cas9 II protein.

Techniques: Fluorescence, Microscopy, Construct, CRISPR

Journal: bioRxiv

Article Title: Nucleolar stress induced the formation of a nucleolar stress body via the NOSR-1/NUMR-1 axis in Caenorhabditis elegans

doi: 10.1101/2024.03.05.583293

Figure Lengend Snippet:

Article Snippet: Addgene plasmid #47549 was used to express Cas9 II protein.

Techniques: CRISPR